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Objective To investigate the expression of flotillin-1 in cervical cancer,and molecular mechanism and relationship between flotillin-1 and lymph node metastasis.Methods Real-time polymerase chain reaction (RT-PCR),Western blot and immunohistochemistry were used to detect the expression of flotillin-1 in cervical cancer cells and tissues,and detect the role of flotillin-1 in cervical cancer metastasis and the possible mechanism by over-expression and interference.Results The protein and mRNA expression of Flotillin-1 were significantly upregnlated in cervical cancer cell lines and cancer tissues.The elevated expression of flotillin-1 protein in early-stage cervical cancers was significantly associated with pelvic lymph node metastasis (P ＜0.01).Moreover,flotillin-1 up-and down-regulations remarkably affected the motility and invasion of cervical cancer cells through epithelial-mesenchymal transition (EMT) regulated by the Wnt//β-catenin and nuclear factor-κB (NF-κB) pathways.Conclusions Flotillin-1 regulates the EMT process through the catenin beta Wnt and NF-κB signaling pathway,and promotes the metastasis of cervical cancers.The flotillin-1 expression profile serves as novel independent predictor of lymph node metastasis in early-stage cervical cancers.
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Objective:To investigate the apoptosis by thiocoraline in combination with cisplatin on cervical cell line c4-1 and Hela and its mechanism. Methods:Different concentrations of thiocoraline and cisplatin alone or in combination used for cultured c4-1 and Hela cells. The inhibition of cell proliferation was detected by MTT assay,and the apoptosis was detected by Hoechst 33342 and AO-EB staining. The expressions of the protein Bax,Bcl2,p53,p21 were detected by Western blot. The cell cycle of c4-1 and Hela cells were detected by Flow cytometry. Results: The cell proliferation activity of c4-1 and HeLa cells were inhibited obviously by different concentrations of thiocoraline and cisplatin alone or in combination. The inhibition effect was in a dose-dependent manner. Meanwhile, when the concentrations of thiocoraline and cisplatin were 10 nmol/L and 4 μmol/L, respectivity the inhibitory effect was most significant (P<0. 05);Hoechst 33342 and AO-EB staining showed that the cell apoptosis effect was more obvious on thiocoraline in combination with cisplatin. The Western blot showed that the expressions of the protein Bax,p53,p21 were significantly up-regulated, and the Bcl2 was significantly down-regulated. Conclusion:Thiocoraline combination with cisplatin could up-regulate the expression of the protein Bax,p53,p21 and down-regulation the expression of Bcl2 to inhibition the proliferation and promotion apoptosis of cervical cell line c4-1 and Hela.
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Objective To investigate the expression and clinical significance of SETDB1 in human ovarian carcinoma.Methods 44 ovarian carcinoma tissues and matched tumor-adjacent tissues were collected from January,2010 to December,2012.The mRNA expression of SETDB1 was detected by qRT-PCR,and the protein expression of SETDB1 was detected by immunohistochemistry.The correlation between SETDB1 and clinic pathological features was analyzed by chi-square test,and the Kaplan-Meier survival curves were drawn to describe the prognostic effects of SETDB1 expression.Results The expression of SETDB1 was up-regulated in ovarian carcinoma tissues compared to those matched tumor-adjacent tissues(P ＜ 0.05).Positive expression of SETDB1 was associated with lymphatic metastasis (P ＜ 0.05) and advanced FIGO stage (Ⅲ + Ⅳ,P ＜ 0.05).Both the 3-year overall survival rate and tumor-free survival rate were lower in SETDB1 positive expression group than SETDB1 negative expression group (P ＜ 0.05).Conclusion Positive expression of SETDB1 in human ovarian carcinoma is related to the poor prognosis.SETDB1 has a potential to predict the prognosis for ovarian carcinoma patients.